Deiana et al. 1999

Deiana AM, Cau A, Coluccia E, Cannas R, Milia A, Salvadori S, Libertini A. 1999. Genome size and AT-DNA content in thirteen species of Decapoda. In: Crustaceans and the Biodiversity Crisis, Schram FR, von Vaupel Klein JC (eds.) Koninklijke Brill NV Leiden, The Netherlands, pp. 981-985.

These authors report genome sizes for 12 species of decapods within one monophyletic lineage, plus one additional species considered as an outgroup. Decapod genome sizes cover a broad range, from about 1.3 to 22.6 pg, with a mode of about 2 or 3 pg, based on a previous study (Lécher et al., 1995). This study used flow cytometry, using Propidium Iodide (PI) for genome size estimation and DAPI for AT-content. Species identification was simplified by the use of primarily commercially-derived species, though non-Mediterranean species were identified by reference to Holthius (1991).

The tissue used in flow cytometry was either gill or antennal gland; both tissues provided low-variability cells. All samples were coprepared with European Eel (Anguilla anguilla) red blood cells. Samples were run with PI first, then washed and fixed with 70% ethanol and stained with DAPI; in all cases a count of at least 2500 cells was obtained.