Thursday, February 28, 2008

Doležel 1991

Doležel J. 1991. Flow cytometric analysis of nuclear DNA content in higher plants. Phyochemical Analysis 2: 143-154.

This author reviews the state-of-the-art of flow cytometry as applied to plants in 1991. A useful and detailed technical discussion preceedes advice for choosing stains and preparing samples, along with interesting historical notes on the development of stains and proceedures. Notably, this author strongly recommends co-prepared samples to avoid errors associated with shifts between samples and the use of only intercalating dyes such as Propidium Iodide and Ethidium Bromide when estimating genome size.

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