Carbonari M, Mancaniello D, Tedesco T, Fiorilli M. 2008. Flow acetone-staining technique: a highly efficient procedure for the simultaneous analysis of DNA content, cell morphology, and immunophenotype by flow cytometry. Cytometry Part A 73A: 168-174.
These authors have developed a cell-handling procedure that allows simultaneous examination of nuclear DNA content, cell morphology, and some aspects of membrane-associated immunoproteins in mammalian cells from culture. The foundation of this technique is the use of 80% acetone at 8°C as a fixative during the initial stages of cell processing for flow cytometry.
These authors tested a range of fixative agents and conditions, and found that while previous work had rejected acetone as a useful fixative, careful control of temperature at each stage of fixation and preparation allows the useful properties of acetone to outweigh its negative qualities for this type of work.
The application of this technique to my own work seems very limited, as I am not interested in immunochemistry, only in nuclear DNA content.
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