Brockhoff G, Fleischmann S, Meier A, Wachs F-P, Hofstaedter F, Knuechel R. 1999. Use of a mechanical dissociation device to improve standardization of flow cytometric cytokeratin DNA measurements of colon carcinomas. Cytometry (Communications in Clinical Cytometry) 38: 184-191.
These authors evaluated the use of a Medimachine (DAKO Diagnostica GmbH) to dissociate cells from specimens of colon tumours in a large number of patients. They compared flow cytometry results from specimens sliced by the Medimachine’s “little crossed blades” to manual dissociation using scalpels. In general, the Medimachine produced a higher fraction of useable cells in flow cytometry, and was more consistent in an interlaboratory comparison.
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