Sunday, May 18, 2008

Singh 1998

Singh NP. 1998. A rapid method for the preparation of single-cell suspensions from solid tissues. Cytometry 31: 229-232.

This author evaluated the use of a tissue press (BioSpec Product, Inc.) to produce cell suspensions suitable for examination of DNA double-stranded breaks in rat brain tissue. The use of manual cell dissociation with forceps and scalpels was compared to the device, and the device was found to be highly effective and efficient at producing cell suspensions.

This device is not expensive (approx. $30) and looks like it may be useful for my own work with preparing cell suspensions for flow cytometry.

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